Review



dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences  (GenScript corporation)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    GenScript corporation dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences
    Dna Coding Sequences (Cds) Of The Candidate Immunogens And The Mcherry Reporter, And The Flanking Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences - by Bioz Stars, 2026-06
    90/100 stars

    Images



    Similar Products

    dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences  (GenScript corporation)
    90
    GenScript corporation dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences
    Dna Coding Sequences (Cds) Of The Candidate Immunogens And The Mcherry Reporter, And The Flanking Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    dna coding sequences (cds) of the candidate immunogens and the mcherry reporter, and the flanking sequences - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    coding sequence (cds) of candidates  (GenScript corporation)
    90
    GenScript corporation coding sequence (cds) of candidates
    (A) mScarlet expression in the 6 indicated KOs, rescued with their respective V5-tagged <t>protein-coding</t> <t>sequence</t> <t>(CDS),</t> or with an empty vector. Each data point is an individual clone (n=3 clones per KO). (B) Hygromycin resistance after rescue. One representative clone is shown for each KO. (C) Dazl expression after rescue (n=3 rescued clones per KO). (D) Western blot analysis of DAZL and the indicated V5-tagged CDS. (E) RT-qPCR assays showing the expression changes of Dazl , differentiation ( Fgf5 ) and pluripotency ( Prdm14, Pou5f1 ) markers upon spontaneous differentiation after withdrawal of LIF. (F) MeDIP assay showing the relative levels of 5mC at the Dazl promoter for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO). (G) LUMA assay showing the changes in the global level of DNA methylation for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO). (H and I) LC-MS/MS assay showing the changes in the global level of 5mdC/C (H) and 5hmdC/C (I) for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO).
    Coding Sequence (Cds) Of Candidates, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coding sequence (cds) of candidates/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    coding sequence (cds) of candidates - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) mScarlet expression in the 6 indicated KOs, rescued with their respective V5-tagged protein-coding sequence (CDS), or with an empty vector. Each data point is an individual clone (n=3 clones per KO). (B) Hygromycin resistance after rescue. One representative clone is shown for each KO. (C) Dazl expression after rescue (n=3 rescued clones per KO). (D) Western blot analysis of DAZL and the indicated V5-tagged CDS. (E) RT-qPCR assays showing the expression changes of Dazl , differentiation ( Fgf5 ) and pluripotency ( Prdm14, Pou5f1 ) markers upon spontaneous differentiation after withdrawal of LIF. (F) MeDIP assay showing the relative levels of 5mC at the Dazl promoter for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO). (G) LUMA assay showing the changes in the global level of DNA methylation for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO). (H and I) LC-MS/MS assay showing the changes in the global level of 5mdC/C (H) and 5hmdC/C (I) for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO).

    Journal: bioRxiv

    Article Title: A genome-wide knock-out screen for actors of epigenetic silencing reveals new regulators of germline genes and 2-cell like cell state

    doi: 10.1101/2021.05.03.442415

    Figure Lengend Snippet: (A) mScarlet expression in the 6 indicated KOs, rescued with their respective V5-tagged protein-coding sequence (CDS), or with an empty vector. Each data point is an individual clone (n=3 clones per KO). (B) Hygromycin resistance after rescue. One representative clone is shown for each KO. (C) Dazl expression after rescue (n=3 rescued clones per KO). (D) Western blot analysis of DAZL and the indicated V5-tagged CDS. (E) RT-qPCR assays showing the expression changes of Dazl , differentiation ( Fgf5 ) and pluripotency ( Prdm14, Pou5f1 ) markers upon spontaneous differentiation after withdrawal of LIF. (F) MeDIP assay showing the relative levels of 5mC at the Dazl promoter for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO). (G) LUMA assay showing the changes in the global level of DNA methylation for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO). (H and I) LC-MS/MS assay showing the changes in the global level of 5mdC/C (H) and 5hmdC/C (I) for 6 candidates KO, compared to the parental DASH mES cells (n=3 clones per KO).

    Article Snippet: For rescue experiments, the coding sequence (CDS) of candidates was synthesized (for Bend3, Spop, and Zbtb14) (GenScript), amplified from cDNA ( Kdm5c ), or obtained from colleagues (for Mcm3ap , kindly shared by Prof. N. Sakaguchi, ).

    Techniques: Expressing, Sequencing, Plasmid Preparation, Clone Assay, Western Blot, Quantitative RT-PCR, Methylated DNA Immunoprecipitation, DNA Methylation Assay, Liquid Chromatography with Mass Spectroscopy

    (A) RT-qPCR analysis of the expression of 2C-specific genes in Spop KO, Mcm3ap KO, Kdm5c KO, and Zbtb14 KO, compared to the parental DASH mES cells (n=3 clones per KO). (B) Western blot analysis of ZSCAN4 and MERVL-Gag for Spop KO, Mcm3ap KO, Kdm5c KO, Zbtb14 KO, and parental DASH mES cells cultured in Serum or 2i. (C) RT-qPCR analysis of the expression of 2C-specific genes for Spop KO, Mcm3ap KO, Kdm5c KO, and Zbtb14 KO, transfected with either an empty plasmid or their respective CDS, compared to the parental DASH mES cells (n=3, clones per KO). (D) Western blot analysis of V5, ZSCAN4, and MERVL-Gag in 4 candidates KO, transfected with an empty plasmid or their respective V5-tagged CDS. (E) Model for role of novel epigenetic factors ZBTB14, KDM5C, SPOP, MCM3AP, BEND3, and KMT2D in the regulation of germline genes, and 2 cell-like cell state.

    Journal: bioRxiv

    Article Title: A genome-wide knock-out screen for actors of epigenetic silencing reveals new regulators of germline genes and 2-cell like cell state

    doi: 10.1101/2021.05.03.442415

    Figure Lengend Snippet: (A) RT-qPCR analysis of the expression of 2C-specific genes in Spop KO, Mcm3ap KO, Kdm5c KO, and Zbtb14 KO, compared to the parental DASH mES cells (n=3 clones per KO). (B) Western blot analysis of ZSCAN4 and MERVL-Gag for Spop KO, Mcm3ap KO, Kdm5c KO, Zbtb14 KO, and parental DASH mES cells cultured in Serum or 2i. (C) RT-qPCR analysis of the expression of 2C-specific genes for Spop KO, Mcm3ap KO, Kdm5c KO, and Zbtb14 KO, transfected with either an empty plasmid or their respective CDS, compared to the parental DASH mES cells (n=3, clones per KO). (D) Western blot analysis of V5, ZSCAN4, and MERVL-Gag in 4 candidates KO, transfected with an empty plasmid or their respective V5-tagged CDS. (E) Model for role of novel epigenetic factors ZBTB14, KDM5C, SPOP, MCM3AP, BEND3, and KMT2D in the regulation of germline genes, and 2 cell-like cell state.

    Article Snippet: For rescue experiments, the coding sequence (CDS) of candidates was synthesized (for Bend3, Spop, and Zbtb14) (GenScript), amplified from cDNA ( Kdm5c ), or obtained from colleagues (for Mcm3ap , kindly shared by Prof. N. Sakaguchi, ).

    Techniques: Quantitative RT-PCR, Expressing, Clone Assay, Western Blot, Cell Culture, Transfection, Plasmid Preparation

    (A) RT-qPCR analysis of the expression of 2C-specific genes in DASH mES cells cultured in 2i, Bend3 KO, and Kmt2d KO. (B) Western blot analysis of ZSCAN4 and MERVL-Gag for Bend3 KO, Kmt2d KO, and parental DASH mES cells cultured in Serum or 2i. (C) RT-qPCR analysis of the expression of 2C-specific genes for Bend3 KO (n=3, clones), transfected with an empty plasmid or their respective CDS, compared to the parental DASH mES cells. (D) Western blot analysis of ZSCAN4 and MERVL-Gag for Kdm5c KO clones, transfected with an empty plasmid, or a wild-type Kdm5c CDS, or a catalytically dead Kdm5c CDS (H514A). (E) RT-qPCR analysis of the expression of 2C-specific genes for Kdm5c KO clones, transfected with an empty plasmid, or a wild-type Kdm5c CDS, or a catalytically dead Kdm5c CDS (H514A).

    Journal: bioRxiv

    Article Title: A genome-wide knock-out screen for actors of epigenetic silencing reveals new regulators of germline genes and 2-cell like cell state

    doi: 10.1101/2021.05.03.442415

    Figure Lengend Snippet: (A) RT-qPCR analysis of the expression of 2C-specific genes in DASH mES cells cultured in 2i, Bend3 KO, and Kmt2d KO. (B) Western blot analysis of ZSCAN4 and MERVL-Gag for Bend3 KO, Kmt2d KO, and parental DASH mES cells cultured in Serum or 2i. (C) RT-qPCR analysis of the expression of 2C-specific genes for Bend3 KO (n=3, clones), transfected with an empty plasmid or their respective CDS, compared to the parental DASH mES cells. (D) Western blot analysis of ZSCAN4 and MERVL-Gag for Kdm5c KO clones, transfected with an empty plasmid, or a wild-type Kdm5c CDS, or a catalytically dead Kdm5c CDS (H514A). (E) RT-qPCR analysis of the expression of 2C-specific genes for Kdm5c KO clones, transfected with an empty plasmid, or a wild-type Kdm5c CDS, or a catalytically dead Kdm5c CDS (H514A).

    Article Snippet: For rescue experiments, the coding sequence (CDS) of candidates was synthesized (for Bend3, Spop, and Zbtb14) (GenScript), amplified from cDNA ( Kdm5c ), or obtained from colleagues (for Mcm3ap , kindly shared by Prof. N. Sakaguchi, ).

    Techniques: Quantitative RT-PCR, Expressing, Cell Culture, Western Blot, Clone Assay, Transfection, Plasmid Preparation